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Veterinary Pathology, Vol 22, Issue 4 327-332, Copyright © 1985 by American College of Veterinary Pathologists

ARTICLES

Histochemical demonstration of copper and copper-associated protein in the canine liver

L. P. Thornburg, M. Beissenherz, M. Dolan and M. F. Raisbeck

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.




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Copyright © 1985 by the American College of Veterinary Pathologists.