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Veterinary Pathology, Vol 33, Issue 3 341-343, Copyright © 1996 by American College of Veterinary Pathologists


ARTICLES

Immunohistochemical detection of virulence-associated antigens of Rhodococcus equi in pulmonary lesions of foals

H. Madarame, S. Takai, N. Morisawa, M. Fujii, D. Hidaka, S. Tsubaki and Y. Hasegawa
Department of Laboratory Animal Science, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan.

Rhodococcus equi was isolated from the lungs of six foals with bronchopneumonia. All isolates expressed 15-17-kd antigens by immunoblot analysis and contained a virulence-associated plasmid of 85 or 90 kb. Immunohistochemically, R. equi from all pulmonary lesions showed the expression of 15-17-kd antigens mainly in the phagocytic cells. The specific monoclonal antibody to 15-17-kd antigens of R. equi (MAb 10G5) may be an aid in the diagnosis of R. equi-induced pneumonia.


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Copyright © 1996 by the American College of Veterinary Pathologists.