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Veterinary Pathology, Vol 35, Issue 3 159-167, Copyright © 1998 by American College of Veterinary Pathologists

ARTICLES

In situ hybridization with riboprobes: an overview for veterinary pathologists

C. Brown
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602-7388, USA.

In situ hybridization using nonradioactively-labeled RNA probes is a technique that combines understanding of basic molecular biology and histopathologic interpretation. Recombinant or PCR technology can be used to produce probes that hybridize with a wide variety of cellular genes and infectious agents. Hybridization conditions can be optimized for each probe/target combination.
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