Veterinary Pathology, Vol 36, Issue 4 292-300, Copyright © 1999 by American College of Veterinary Pathologists

ARTICLES

Prognostic factors for treated canine malignant lymphoma

M. Kiupel, E. Teske and D. Bostock
Institut fur Veterinar-Pathologie der Freien Universitat Berlin, Germany.

The aim of this study was to investigate the prognostic importance of different clinical, immunohistologic and tumor proliferation characteristics in dogs with malignant lymphoma treated with chemotherapy. From 74 dogs with malignant lymphoma at least one enlarged peripheral lymph node was taken for biopsy before chemotherapy following a standardized protocol (vincristine, cyclophosphamide, prednisolone, doxorubicin, and L-asparaginase). The variables evaluated as prognostic factors were age, sex, and tumor stage, as well as histomorphologic grade (Kiel classification, Working Formulation), immunophenotype (using markers for CD3 and CD79a), and cell proliferation (Ki-67, proliferation cell nuclear antigen, mitotic index, and argyrophil nucleolar organizer regions [AgNORs]) in extirpated lymph nodes. All markers were used on routinely formalin-fixed, paraffin-embedded tissues. The AgNORs were assessed qualitatively, based on the AgNOR pattern distribution, and quantitatively using image analysis and routine counting. In both univariate and multivariate survival analyses, AgNORs were a valuable prognostic marker for the treatment of canine malignant lymphomas. Based on the results of the multivariate analysis longer survival time correlated with a B-cell type, a larger mean AgNOR area, a larger total AgNOR area, a shorter distance between two AgNORs, and a smaller AgNOR area to nucleus ratio. Longer disease-free survival time correlated with a smaller number of AgNORs per nucleus, a larger mean AgNOR area, a larger maximal AgNOR area, and a larger total AgNOR area. This study clearly demonstrates the additional benefit of the use of AgNORs in predicting treatment outcome in dogs with malignant lymphoma.

This article has been cited by other articles:

				L. F. P. Silva, B. E. Etchebarne, M. S. Weber Nielsen, J. S. Liesman, M. Kiupel, and M. J. VandeHaar Intramammary Infusion of Leptin Decreases Proliferation of Mammary Epithelial Cells in Prepubertal Heifers J Dairy Sci, August 1, 2008; 91(8): 3034 - 3044. [Abstract] [Full Text] [PDF]

				M. A. Higgins, J. H. Rossmeisl, G. K. Saunders, S. Hayes, and M. Kiupel B-Cell Lymphoma in the Peripheral Nerves of a Cat Vet. Pathol., January 1, 2008; 45(1): 54 - 57. [Abstract] [Full Text] [PDF]

				J. D. Frank, S. B. Reimer, P. H. Kass, and M. Kiupel Clinical Outcomes of 30 Cases (1997 2004) of Canine Gastrointestinal Lymphoma J. Am. Anim. Hosp. Assoc., November 1, 2007; 43(6): 313 - 321. [Abstract] [Full Text] [PDF]

				J. D. Webster, V. Yuzbasiyan-Gurkan, R. A. Miller, J. B. Kaneene, and M. Kiupel Cellular Proliferation in Canine Cutaneous Mast Cell Tumors: Associations with c-KIT and Its Role in Prognostication Vet. Pathol., May 1, 2007; 44(3): 298 - 308. [Abstract] [Full Text] [PDF]

				C. I. Kaiser, J. L. Fidel, M. Roos, and B. Kaser-Hotz Reevaluation of the University of Wisconsin 2-Year Protocol for Treating Canine Lymphosarcoma J. Am. Anim. Hosp. Assoc., March 1, 2007; 43(2): 85 - 92. [Abstract] [Full Text] [PDF]

				J. Meyer, J. DeLay, and D. Bienzle Clinical, laboratory, and histopathologic features of equine lymphoma. Vet. Pathol., November 1, 2006; 43(6): 914 - 924. [Abstract] [Full Text] [PDF]

				J. Pinard, C. R. Wagg, C. Girard, M. Kiupel, and C. Bedard Histiocytic sarcoma in the tarsus of a cat. Vet. Pathol., November 1, 2006; 43(6): 1014 - 1017. [Abstract] [Full Text] [PDF]

				V. E. Valli, W. Vernau, L.-P de Lorimier, P. S. Graham, and P. F. Moore Canine Indolent Nodular Lymphoma Vet. Pathol., May 1, 2006; 43(3): 241 - 256. [Abstract] [Full Text] [PDF]

				P. Vajdovich, R. Psader, Z. A. Toth, and E. Perge Use of the Argyrophilic Nucleolar Region Method for Cytologic and Histologic Examination of the Lymph Nodes in Dogs Vet. Pathol., July 1, 2004; 41(4): 338 - 345. [Abstract] [Full Text] [PDF]

				C. Fournel-Fleury, F. Ponce, P. Felman, A. Blavier, C. Bonnefont, L. Chabanne, T. Marchal, J. L. Cadore, I. Goy-Thollot, D. Ledieu, et al. Canine T-cell Lymphomas: A Morphological, Immunological, and Clinical Study of 46 New Cases Vet. Pathol., January 1, 2002; 39(1): 92 - 109. [Abstract] [Full Text] [PDF]