| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Veterinary Pathology (JY, 1 HY, ,1, MI, MK, TK, SS), Veterinary Anatomy (YT), Veterinary Surgery (FO), Veterinary Internal Medicine (TM), College of Agriculture, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka, Japan and Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan (MT)
The monoclonal antibody AM-3K, which was developed using human pulmonary macrophages as the immunogen, immunocytochemically labels most human macrophages except for blood monocytes and dendritic cell populations. AM-3K also shows cross-reactivity in some animal species. To evaluate the usefulness of AM-3K, the present study investigated the detailed distribution of AM-3K–immunopositive macrophages in normal and diseased tissues of dogs, cats, horses, cattle, pigs, and rabbits. Zamboni's solution–fixed, paraffin-embedded sections were the most available for the immunocytochemistry with AM-3K. In all animal species examined, AM-3K labeled most macrophages in splenic red pulp, lymph node sinuses and thymus, and tissue macrophages in the interstitium of various organs and sites such as the kidneys, lungs, heart, pancreas, intestines, and skin. Alveolar macrophages and perivascular microglial cells were also immunoreactive for AM-3K. Interestingly, Kupffer cells of dogs, cats, and horses were labeled for AM-3K, but those of cattle, pigs, and rabbits were not. Furthermore, in tumor tissues and inflammatory lesions such as liver fibrosis and encephalomalacia that were obtained from dogs, infiltrating macrophages were stained with AM-3K, but not all infiltrating macrophages reacted to AM-3K. In addition, only 30–50% of pulmonary and peritoneal macrophages obtained from cats and dogs were reactive for AM-3K. AM-3K did not react with blood monocytes, dendritic cell populations, and osteoclasts. These observations indicate that AM-3K specifically labels most exudate and tissue macrophages in the animal species examined. However, the expression of antigens recognized by AM-3K on macrophages may be dependent on differential maturation stages or different functions evoked by some conditions. AM-3K immunoreaction products were seen on the cytoplasmic membrane of macrophages by immunoelectron microscopy. AM-3K would be useful for detection of macrophage populations in the animal species examined here.
Key words: Domestic animals; immunocytochemistry; macrophage; macrophage-specific antibody.
Request reprints from Dr. J. Yamate, Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka 599–8531 (Japan). E-mail: yamate{at}vet.osakafu-u.ac.jp.
This article has been cited by other articles:
 |
|
 |
|
  Y. Komohara, J. Hirahara, T. Horikawa, K. Kawamura, E. Kiyota, N. Sakashita, N. Araki, and M. Takeya AM-3K, an Anti-macrophage Antibody, Recognizes CD163, a Molecule Associated with an Anti-inflammatory Macrophage Phenotype J. Histochem. Cytochem., July 1, 2006; 54(7): 763 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yamate, Y. Machida, M. Ide, M. Kuwamura, T. Kotani, O. Sawamoto, and J. LaMarre Cisplatin-Induced Renal Interstitial Fibrosis in Neonatal Rats, Developing as Solitary Nephron Unit Lesions Toxicol Pathol, February 1, 2005; 33(2): 207 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Rogers, C. K. Mathiason, and E. A. Hoover Immunohistochemical Localization of Feline Immunodeficiency Virus Using Native Species Antibodies Am. J. Pathol., October 1, 2002; 161(4): 1143 - 1151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yamate, K. Sato, M. Ide, M. Nakanishi, M. Kuwamura, S. Sakuma, and S. Nakatsuji Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats Vet. Pathol., May 1, 2002; 39(3): 322 - 333. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
