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p53 Gene Mutations Occurring in Spontaneous Benign and Malignant Mammary Tumors of the Dog

Department of Experimental Pathology, Comparative Toxicology Laboratories, School of Veterinary Medicine, Azabu University, Kanagawa, Japan (TMuto, SW, TMasaoka); Department of Pathology, School of Medicine, The Jikei University, Minatoku, Tokyo, Japan (SW, HT, SU); and Department of Pathology, School of Medicine, Kyorin University, Mitakashi, Tokyo, Japan (SW, SM, MF)

Sixty-three cases of benign and malignant canine mammary tumors were analyzed to define the alteration of exons 5–8 for the p53 tumor suppressor gene using polymerase chain reaction direct sequence analysis with paraffin-embedded tissues. Four missense mutations were found in 38 benign mammary tumors (11%), and five missense (one tumor had two missense mutations) and one nonsense mutations were found in 25 mammary carcinomas (20%). These data suggest that the p53 gene alterations might be initiated at an early stage of canine mammary carcinogenesis and p53 mutations might be associated with malignancy. However, there was no evidence of any relationship between the p53 alterations and the histologic types of tumors or breeds of dogs.

Key words: Dogs; mammary neoplasms; mutation; p53; tumor suppressor gene.

Address reprint requests to Dr. S. Wakui, Comparative Toxicology Laboratories, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501 (Japan). E-mail: wakui{at}azabu-u.ac.jp.

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