This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ramos-Vara, J. A. Articles by Johnson, G. C. Search for Related Content PubMed PubMed Citation Articles by Ramos-Vara, J. A. Articles by Johnson, G. C.

Immunohistochemical Characterization of Canine Hyperplastic Hepatic Lesions and Hepatocellular and Biliary Neoplasms with Monoclonal Antibody Hepatocyte Paraffin 1 and a Monoclonal Antibody to Cytokeratin 7

Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO

Immunostaining with monoclonal antibody (MoAb) hepatocyte paraffin 1 (Hep Par 1) and an MoAb to cytokeratin 7 (CK7) was performed on 105 formalin-fixed, paraffin-embedded canine hyperplastic and neoplastic hepatic lesions. Hep Par 1 was detected in 12/12 hyperplastic nodules, 17/17 hepatocellular adenomas, and 37/40 hepatocellular carcinomas. The staining was disseminated, granular, and cytoplasmic. This antibody did not react with normal or neoplastic biliary epithelium. Other hepatic tumors or tumors metastatic to the liver did not bind Hep Par 1 except one metastatic intestinal carcinoma. MoAb to CK 7 stained all hyperplastic biliary epithelium and benign cholangiocellular tumors (5/5) and 14/18 cholangiocellular carcinomas. One hepatocellular carcinoma had cells positive for both Hep Par 1 and CK 7. Liver was the only normal tissue tested that reacted with MoAb Hep Par 1. Only five nonhepatic tumors (one adrenocortical carcinoma, one interstitial cell tumor of the testis, one melanoma, and two salivary adenocarcinomas) of 277 tumors tested had focal/multifocal staining for Hep Par 1. Prolonged fixation did not alter the staining with Hep Par 1. We conclude that Hep Par 1 is a specific and sensitive marker for canine hepatocellular tumors and allows distinction between hepatocellular and biliary neoplasms.

Key words: Biliary tumors; cytokeratin 7; dogs; hepatocellular tumors; hepatocyte paraffin 1; immunohistochemistry.

Request reprints from Dr. J. A. Ramos-Vara, Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, PO Box 6023, Columbia, MO 65205 (USA). E-mail: ramosj{at}missouri.edu.

This article has been cited by other articles:

				J. A. Ramos-Vara, M. Kiupel, T. Baszler, L. Bliven, B. Brodersen, B. Chelack, S. Czub, F. D. Piero, S. Dial, E.J. Ehrhart, et al. Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories J Vet Diagn Invest, July 1, 2008; 20(4): 393 - 413. [Abstract] [Full Text] [PDF]

				J. A. Ramos-Vara, M. A. Miller, and G. C. Johnson Usefulness of Thyroid Transcription Factor-1 Immunohistochemical Staining in the Differential Diagnosis of Primary Pulmonary Tumors of Dogs Vet. Pathol., May 1, 2005; 42(3): 315 - 320. [Abstract] [Full Text] [PDF]

				J. A. Ramos-Vara, M. A. Miller, M. Boucher, A. Roudabush, and G. C. Johnson Immunohistochemical Detection of Uroplakin III, Cytokeratin 7, and Cytokeratin 20 in Canine Urothelial Tumors Vet. Pathol., January 1, 2003; 40(1): 55 - 62. [Abstract] [Full Text] [PDF]

				J. A. Ramos-Vara, M. A. Miller, G. C. Johnson, and L. W. Pace Immunohistochemical Detection of Thyroid Transcription Factor-1, Thyroglobulin, and Calcitonin in Canine Normal, Hyperplastic, and Neoplastic Thyroid Gland Vet. Pathol., July 1, 2002; 39(4): 480 - 487. [Abstract] [Full Text] [PDF]