This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taniyama, H. Articles by Taya, K. Search for Related Content PubMed PubMed Citation Articles by Taniyama, H. Articles by Taya, K.

Immunohistochemical Detection of Inhibin-, -ßB, and -ßA Chains and 3ß-hydroxysteroid Dehydrogenase in Canine Testicular Tumors and Normal Testes

Departments of Veterinary Pathology (HT, KH, KN, NY, YK), Veterinary Obstetrics and Gynecology (KN), and Veterinary Surgery (YI, TN), School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan; and Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan (YT, GW, KT)

Immunohistochemical detection of inhibin-, -ßA and -ßB chains and 3ß-hydroxysteroid dehydrogenase (HSD) was carried out on primary testicular tumors from 15 dogs and normal testes from three adult dogs. Histopathologically, the tumors were composed of three types: Leydig cell tumors in five dogs, Sertoli cell tumors in five dogs, and seminoma in five dogs. In normal testes, immunostaining against inhibin-, -ßA, and -ßB chains and 3ß-HSD revealed positive reactivity in the cytoplasm of Leydig cells. In testicular tumors, immunoreactive cells against inhibin-, -ßA, and -ßB chains and 3ß-HSD were localized in all Leydig cell tumors but not in any Sertoli cell tumors or seminomas. The results of radioimmunoassay for plasma inhibin in dogs with Leydig cell tumors showed higher concentrations than those in dogs with Sertoli cell tumors and seminomas and those in normal dogs. The concentration of inhibin in the plasma was markedly decreased by the surgical removal of the Leydig cell tumor in one dog. Our findings suggest that inhibin is synthesized by normal and neoplastic Leydig cells in the canine testis, and the secreted inhibin may be inhibin A and inhibin B.

Key words: Dogs; immunohistochemistry; inhibin; testis; tumor.

Request reprints from Dr. H. Taniyama, Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midorimachi 582, Ebetsu, Hokkaido 069-8501 (Japan).

This article has been cited by other articles:

				Z. A. Radi, D. L. Miller, and M. E. Hines II Rete Testis Mucinous Adenocarcinoma in a Dog Vet. Pathol., January 1, 2004; 41(1): 75 - 78. [Abstract] [Full Text] [PDF]