This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Higdon, H. L. Articles by Spitzer, J. C. Search for Related Content PubMed PubMed Citation Articles by Higdon, H. L., III Articles by Spitzer, J. C.

Streptozocin-induced Pancreatic Islet Destruction in Beef Cows

Abstract

Streptozocin (STZ) induces diabetes mellitus in sheep and pigs. To test the effect of STZ in cattle, cows were given 75–150 mg STZ per kilogram of body weight. Cows receiving 150 mg/kg required euthanasia within 24 hours after infusion because of the severe systemic effects of STZ. Seven cows receiving doses of 100 mg/kg had mild to marked decrease in islet immunoreactivity for insulin and in pancreatic islet density and mild to severe tubulointerstitial nephritis. Two cows receiving 75 and 85 mg/kg STZ regained their ability to produce insulin and return blood glucose to basal levels. One cow given 100 mg/kg STZ developed insulin insufficiency consistent with type I diabetes mellitus. These findings demonstrate the susceptibility of the bovine pancreas to STZ; however, severe systemic complications were encountered. Alternative dosages and methodologies should be considered in future attempts to induce diabetes in cattle using STZ.

Key words: Cattle; diabetes; experimental disease; immunohistochemistry; insulin; pancreas; streptozocin.

Request reprints from Dr. H. L. Higdon III, Reproductive Endocrinology and Infertility, Greenville Hospital System, 890 West Faris Road, Suite 470, Greenville, SC 29605-5601 (USA). E-mail: lhigdon{at}ghs.org.