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The Ribonuclease Protection Assay: A Powerful Tool for the Veterinary Pathologist

Millennium Pharmaceuticals, Cambridge, MA

Veterinary pathologists engaged in basic research use a variety of methods to study disease pathogenesis at the light microscopic and submicroscopic (protein and mRNA) levels. The ribonuclease protection assay is a sensitive and accurate method to measure mRNA expression. The major advantages of the assay are that multiple mRNA species can be measured simultaneously in a single total RNA sample and that the assay has relatively high throughput. The major disadvantage is that the assay requires moderate technical skill.

Key words: Chemokine; cytokine; messenger RNA; Northern analysis; receptor; reverse transcription polymerase chain reaction; ribonuclease protection assay.

Request reprints from Dr. J. B. Rottman, Experimental Pathology, Millennium Pharmaceuticals, 640 Memorial Drive, Cambridge, MA 02139 (USA). E-mail: jrottman{at}mpi.com.

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