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Expression of Nitric Oxide Synthase 2 and Tumor Necrosis Factor in Swine Naturally Infected with Actinobacillus pleuropneumoniae

Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Republic of Korea

Nitric oxide synthase 2 (NOS2) and tumor necrosis factor (TNF-) were detected and localized in 15 pigs with naturally occurring pleuropneumonia by use of in situ hybridization with a nonradioactive digoxigenin-labeled cDNA probe. Two cDNA probes 491 and 219 base pairs for NOS2 and TNF-, respectively, were generated by reverse transcription polymerase chain reaction. All 15 pigs infected with Actinobacillus pleuropneumoniae had distinct positive hybridization signals for NOS2 and TNF-. Strong hybridization signals for both NOS2 and TNF- were evident in degenerate alveolar leukocytes bordering zones of coagulative necrosis and in alveolar spaces. NOS2 nucleic acids were detected in neutrophils and macrophages. In situ hybridization of serial sections of lung tissue revealed numerous cells positive for NOS2 and TNF-, suggesting that NOS2 and TNF- expression may play a role in the pathophysiology of pleuropneumonia.

Key words: Actinobacillus pleuropneumoniae; inflammation; in situ hybridization; nitric oxide synthase 2; pigs; pneumonia; polymerase chain reaction; tumor necrosis factor .

Request reprints from Dr. C. Chae, Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Suwon 441–711, Kyounggi-Do (Republic of Korea). E-mail: swine{at}plaza.snu.ac.kr.

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