Vet Pathol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bautista, M. J.
Right arrow Articles by Gómez-Villamandos, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bautista, M. J.
Right arrow Articles by Gómez-Villamandos, J. C.
Vet Pathol 39:84-91 (2002)
© 2002 American College of Veterinary Pathologists

Early Platelet Aggregation as a Cause of Thrombocytopenia in Classical Swine Fever

M. J. Bautista, E. Ruiz-Villamor, F. J. Salguero, P. J. Sánchez-Cordón, L. Carrasco and J. C. Gómez-Villamandos

Departamento de Anatomía y Anatomía Patalógica, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain

Twenty pigs were inoculated with a virulent classical swine fever virus isolate to determine the mechanism responsible for thrombocytopenia using histopathologic, ultrastructural, and immunohistochemical (detection of viral antigens gp55 and FVIII-rag) techniques. In animals euthanatized at 2, 4, and 6 days postinoculation (dpi), clusters of granular material staining positive for FVIII-rag were observed in splenic cords, the marginal zone, hepatic sinusoids, and the perisinusoidal space. Moreover, numerous macrophages in these areas were swollen and displayed an intensely positive granular and cytoplasmic reaction. Cell alterations indicative of platelet activation and secretory and phagocytic activation of resident macrophages were also observed in these sites at 2 and 4 dpi. These results suggest that the thrombocytopenia observed in pigs is caused in the first instance by massive activation and subsequent phagocytosis of platelets secondary to the release of platelet-activating factors by activated macrophages.

Key words: Classical swine fever; pigs; platelets; thrombocytopenia.

Request reprints from Dr. J. C. Gómez-Villamandos, Departamento de Anatomía y Anatomía Patológica Comparadas, Edifficio de Sanidad Animal, Campus Universitario de Rabanales, Carretera de Madrid-Córdoba, Km 396, Córdoba (Spain). E-mail: an1govij{at}uco.es.




This article has been cited by other articles:


Home page
 J. Virol.Home page
D. Stone, Y. Liu, D. Shayakhmetov, Z.-Y. Li, S. Ni, and A. Lieber
Adenovirus-Platelet Interaction in Blood Causes Virus Sequestration to the Reticuloendothelial System of the Liver
J. Virol., May 1, 2007; 81(9): 4866 - 4871.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
P. J. Sanchez-Cordon, A. Nunez, F. J. Salguero, M. Pedrera, M. Fernandez de Marco, and J. C. Gomez-Villamandos
Lymphocyte Apoptosis and Thrombocytopenia in Spleen during Classical Swine Fever: Role of Macrophages and Cytokines
Vet. Pathol., July 1, 2005; 42(4): 477 - 488.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
J. C. Gomez-Villamandos, F. J. Salguero, E. Ruiz-Villamor, P. J. Sanchez-Cordon, M. J. Bautista, and M. A. Sierra
Classical Swine Fever: Pathology of Bone Marrow
Vet. Pathol., March 1, 2003; 40(2): 157 - 163.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American College of Veterinary Pathologists.