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Vet Pathol 39:228-233 (2002)
© 2002 American College of Veterinary Pathologists

Interleukin 4–Producing CD4+ T Cells in the Skin of Cats with Allergic Dermatitis

P. J. Roosje, G. A. Dean, T. Willemse, V. P. M. G. Rutten and T. Thepen

Department of Clinical Sciences of Companion Animals (PJR, 1 TW ,2) and Institute of Infectious Diseases and Immunology (VPMGR), Department of Immunology, Faculty of Veterinary Medicine, and Department of Dermatology/Allergology, Faculty of Medicine, (TT) Utrecht University, Utrecht, the Netherlands; and Department of Microbiology, Pathology & Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC (GAD)

Lesional skin of cats with allergic dermatitis has a cellular infiltrate and a CD4/CD8 ratio comparable to that in humans with atopic dermatitis. CD4+ helper T cells and in particular cells belonging to the Th2 subset play an important role in disease pathogenesis in humans. We investigated the cytokine pattern of CD4+ T cells in situ, with special emphasis on the putative presence of cells producing interleukin 4 (IL4), in cats with allergic dermatitis. Immunohistochemical procedures were used to determine that CD4+ T cells in lesional and nonlesional skin of cats with allergic dermatitis can produce IL4, as occurs in humans. Lesional and nonlesional skin of cats with allergic dermatitis had significantly more IL4+ T cells (P = 0.001) than did skin of healthy control cats. Double staining indicated that all IL4+ cells were positive for pan-T or CD4 markers. Double labeling for mast cell chymase and IL4 stained primarily different cells. Western blotting demonstrated cross-reactivity between the antibody against human IL4 and a feline recombinant IL4. These results indicate that IL4 is primarily produced by CD4+ T cells and is also present in clinically uninvolved skin, indicating a role in the pathogenesis of allergic dermatitis in cats.

Key words: Allergic dermatitis; cats; CD4, chymase; immunohistochemistry; interleukin 4; PCR; skin; T cells; western blotting.

Request reprints from Dr. T. Willemse, Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.154, 3508 TD Utrecht (the Netherlands). E-mail: a.willemse{at}vet.uu.nl.


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