| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA (GDK, KPC, CCB); and Southeast Poultry Research Laboratory, USDA, ARS, Athens, GA (DJK, BSS)
The pathogenesis of six pigeon-origin isolates of Newcastle disease virus (NDV) was investigated in chickens. Four isolates were previously defined as the variant pigeon paramyxovirus 1 (PPMV-1), and two isolates were classified as avian paramyxovirus 1 (APMV-1). Birds inoculated with PPMV-1 isolates were euthanatized, and tissue samples were collected at 2, 5, and 10 days postinoculation (DPI). Birds inoculated with APMV-1 isolates died or were euthanatized, and tissue samples were collected at 2, 4, and 5 DPI. Tissues were examined by histopathology, immunohistochemistry (IHC) for the presence of NDV nucleoprotein, and in situ hybridization (ISH) for the presence of viral mRNA for the matrix gene. Spleen sections were stained by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and by IHC using an anti–active caspase-3 antibody (IHC-Casp) to detect apoptotic cells. Brain sections of PPMV-1–infected birds were examined by IHC to detect T and B lymphocytes and glial fibrillary acidic protein (GFAP). Histologically, birds inoculated with PPMV-1 isolates had marked lesions in the heart and brain. Presence of viral nucleoprotein and viral mRNA in the affected tissues was confirmed by IHC and ISH, respectively. Numerous reactive astrocytes were observed in brain sections stained for GFAP. Among all the isolates, the IHC-Casp demonstrated that apoptosis was very prominent in the ellipsoid-associated cells of the spleen at 2 DPI. Results of the TUNEL assay indicated that apoptotic cells were prominent at 5 DPI and were more randomly distributed. The clinical signs and gross and histopathologic changes observed in the APMV-1–infected birds were characteristic of an extensive infection with highly virulent NDV evident by IHC.
Key words: Apoptosis; avian paramyxovirus 1; chickens; immunohistochemistry; in situ hybridization; Newcastle disease; pathogenesis; pigeon paramyxovirus 1; veterinary virology.
Request reprints from Dr. C. C. Brown, Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7388 (USA). E-mail: corbrown{at}vet.uga.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  L. M. Kim, D. J. King, H. Guzman, R. B. Tesh, A. P. A. T. da Rosa, R. Bueno Jr., J. A. Dennett, and C. L. Afonso Biological and Phylogenetic Characterization of Pigeon Paramyxovirus Serotype 1 Circulating in Wild North American Pigeons and Doves J. Clin. Microbiol., October 1, 2008; 46(10): 3303 - 3310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wakamatsu, D. J. King, B. S. Seal, and C. C. Brown Detection of newcastle disease virus RNA by reverse transcription-polymerase chain reaction using formalin-fixed, paraffin-embedded tissue and comparison with immunohistochemistry and in situ hybridization J Vet Diagn Invest, July 1, 2007; 19(4): 396 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wakamatsu, D. J. King, D. R. Kapczynski, B. S. Seal, and C. C. Brown Experimental Pathogenesis for Chickens, Turkeys, and Pigeons of Exotic Newcastle Disease Virus from an Outbreak in California during 2002-2003. Vet. Pathol., November 1, 2006; 43(6): 925 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Pedersen, D. A. Senne, P. R. Woolcock, H. Kinde, D. J. King, M. G. Wise, B. Panigrahy, and B. S. Seal Phylogenetic Relationships among Virulent Newcastle Disease Virus Isolates from the 2002-2003 Outbreak in California and Other Recent Outbreaks in North America J. Clin. Microbiol., May 1, 2004; 42(5): 2329 - 2334. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
