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Vet Pathol 40:276-282 (2003)
© 2003 American College of Veterinary Pathologists

Evidence of Nitric Oxide Synthase 2 Activity in Swine Naturally Infected with Actinobacillus pleuropneumoniae

W.-S. Cho and C. Chae

Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kwanak-Gu, Seoul, Republic of Korea

Evidence of nitric oxide synthase (NOS) 2 activity was determined by formation of nitrotyrosine (a reaction product of peroxynitrite) and by activation of poly(ADP-ribose) synthetase (PARS) in NOS2-expressed pleuropneumonic lungs from 20 pigs naturally infected with Actinobacillus pleuropneumoniae using immunohistochemistry. Intense immunostaining for nitrotyrosine residue was seen within the lung lesions from A. pleuropneumoniae–infected pigs, but it was minimal in the unaffected parts of the lung from A. pleuropneumoniae–infected pigs and in the normal lung from control pigs. Staining was especially strong in neutrophils and macrophages in the periphery of the lesions and within the alveolar spaces. There was close cell-to-cell correlation when serial sections were examined by immunohistochemistry for NOS2 and nitrotyrosine in each of the 20 lung samples. Expression of PARS was always present within inflammatory lesions but was minimal in the unaffected lung of A. pleuropneumoniae–infected pigs. Macrophages in alveolar spaces frequently exhibited strong staining for PARS. Colocalization of nitrotyrosine and PARS antigen was especially prominent in macrophages in the periphery of lesions. NOS2 expression in pleuropneumonic areas associated with protein nitrosation and PARS suggests that NOS2 is functionally active during infections caused by A. pleuropneumoniae.

Key words: Actinobacillus pleuropneumoniae; immunohistochemistry; nitric oxide; nitric oxide synthase 2; nitrotyrosine; peroxynitrite; pigs; pneumonia; poly(ADP-ribose) synthetase.

Request reprints from Dr. C. Chae, Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul (Republic of Korea). E-mail: swine{at}plaza.snu.ac.kr.




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W.-S. Cho and C. Chae
Expression of Nitric Oxide Synthase 2 and Cyclooxygenase-2 in Swine Experimentally Infected with Actinobacillus pleuropneumoniae
Vet. Pathol., November 1, 2004; 41(6): 666 - 672.
[Abstract] [Full Text] [PDF]

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W.-S. Cho and C. Chae
Expression of Cyclooxygenase-2 and Nitric Oxide Synthase 2 in Swine Ulcerative Colitis Caused by Salmonella typhimurium
Vet. Pathol., July 1, 2004; 41(4): 419 - 423.
[Abstract] [Full Text] [PDF]


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