This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Meyerholz, D. K. Articles by Stabel, T. J. Search for Related Content PubMed PubMed Citation Articles by Meyerholz, D. K. Articles by Stabel, T. J. Social Bookmarking                            What's this?

Comparison of Early Ileal Invasion by Salmonella enterica Serovars Choleraesuis and Typhimurium

Department of Veterinary Microbiology, Iowa State University, Ames, IA (DKM); andPreharvest Food Safety and Enteric Disease Unit, National Animal Disease Center, ARS, USDA, Ames, IA (TJS)

The mechanisms of Salmonella serovar–host specificity are not well defined. Pig ileal loops were used to compare phenotypic differences in early cellular invasion between non–host-adapted Salmonella serovar Typhimurium (SsT) and host-adapted Salmonella serovar Choleraesuis (SsC). By 10 minutes postinoculation, both serovars invaded a small number of M cells, enterocytes, and goblet cells. Multiple SsC organisms (up to 6 per cell) simultaneously invaded M cells, whereas SsT often invaded as one to two organisms per M cell. Internalization of both serovars resulted in vacuoles containing a single bacterium. The follicle-associated epithelium (FAE) of SsC-inoculated loops responded with more filopodia and lamellipodia although exhibiting less cell swelling than SsT. Additionally, SsT showed an enhanced affinity for sites of cell extrusion compared with SsC at 60 minutes. These results suggest: 1) both SsC and SsT exhibit non–cell-specific invasion as early as 10 minutes postinoculation, 2) Salmonella serovars exhibit differences in early invasion of FAE and M cells, and 3) cells undergoing extrusion may provide a site for preferential adherence by SsT and SsC.

Key words: Choleraesuis; DT104; electron microscopy; enterocyte; extrusion; ileum; invasion; Salmonella; swine; Typhimurium.

Request reprints from Dr. T. J. Stabel, NADC, 2300 Dayton Avenue, Ames, IA 50010 (USA). E-mail: tstabel{at}nadc.ars.usda.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F Boyen, F Pasmans, F Van Immerseel, E Donne, E Morgan, R Ducatelle, and F Haesebrouck Porcine in vitro and in vivo models to assess the virulence of Salmonella enterica serovar Typhimurium for pigs Lab Anim, January 1, 2009; 43(1): 46 - 52. [Abstract] [Full Text] [PDF]