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Cyclooxygenase-1 and -2 Expression in Urothelial Carcinomas of the Urinary Bladder in Cows

Department of Pathology and Animal Health, General Pathology Division, Faculty of Veterinary Medicine, Naples University "FEDERICO II," Naples, Italy (GB, VA, PG, FR); and Department of Health and Animal Well-Being, Anatomic Pathology Division, Faculty of Veterinary Medicine, Bari University, Bari, Italy (AP)

Bovine urinary bladder tumors occur frequently in animals suffering from chronic enzootic hematuria because of prolonged ingestion of bracken fern (Pteridium spp.). Cyclooxygenase (COX) genes (COX-1 and COX-2) are known to be involved in the carcinogenesis of human and some animal urothelial tumors. The aim of the present study was to investigate COX-1 and COX-2 expression by immunohistochemical methods in 20 bovine urothelial carcinomas collected at public slaughterhouses from cows that had been suffering from chronic enzootic hematuria. COX-1 immunostaining was identified intracytoplasmically in normal urothelium and in 15 of 20 neoplastic specimens. COX-1 immunosignal in the tumor cells was either absent or weak. COX-2 was also expressed intracytoplasmically in 17 of 20 urothelial carcinomas. Moderate to intense COX-2 labeling was detected in both noninvasive and invasive urothelial carcinomas. Coexpression of both enzyme isoforms was also revealed by confocal laser scanning microscopic investigations. This study indicates that COX-2 is overexpressed in naturally occurring urothelial carcinomas of cows.

Key words: Bracken fern; cows; COX-1; COX-2; confocal laser scanning microscopy; ptaquiloside; urothelial carcinoma.

Request reprints from Prof. Franco Roperto, Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Via Veterinaria 1, 80137 Naples (Italy). E-mail: roperto{at}unina.it.

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