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Immunohistochemical Expression of p53, Fibroblast Growth Factor-b, and Transforming Growth Factor- in Feline Vaccine-associated Sarcomas

Departamento de Patología Animal II, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

Fifty feline sarcomas associated with vaccine-site injection were evaluated to determine the immunohistochemical expression of p53 protein, basic fibroblast growth factor (FGF-b), and transforming growth factor- (TGF-). Forty-one tumors (82%) were fibrosarcomas (FS), eight (16%) were malignant fibrous histiocytomas (MFH), and one (2%) was a chondrosarcoma (CS). Overexpression of p53 protein was observed in the nuclei of tumor cells in 28 (56%) sarcomas; FGF-b expression was found in the cytoplasm of tumor cells in 40 (80%) feline sarcomas, but the staining was more intense in the spindle-shaped cells of FS than in polygonal or round cells of MFH. The single CS faintly expressed FGF-b. The majority of feline vaccine-associated sarcomas (43 of 50, 86%) expressed moderate or intense staining for TGF- in the cytoplasm of tumor cells. Heterogeneous immunolabeling for p53, FGF-b, and TGF- was present in neoplastic, multinucleated giant cells. Intense expression of FGF-b was statistically associated with younger cats (P < 0.01) and with tumors with nodular growth patterns (P = 0.02). In addition, sarcomas negative for p53 protein expressed FGF-b more frequently than did p53-positive tumors (P = 0.04). The frequency of FGF-b immunostaining was significantly higher in sarcomas with intense expression of TGF- (P = 0.05). Immunohistochemical detection of p53 protein, FGF-b, and TGF- suggests that these growth-regulating proteins may play different roles in the development of sarcomas associated with vaccine sites.

Key words: Feline vaccine-associated sarcoma; FGF-b; immunohistochemistry; protein p53; TGF-.

Request reprints from Dr. A. I. Nieto, Departamento de Patología Animal II, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid (Spain).

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