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Vet Pathol 40:713-718 (2003)
© 2003 American College of Veterinary Pathologists
BRIEF COMMUNICATIONS AND CASE REPORTS

Comparison of Tyrosinase-related Protein-2, S-100, and Melan A Immunoreactivity in Canine Amelanotic Melanomas

C. Choi and D. F. Kusewitt

Abstract

Tyrosinase-related protein-2 (TRP-2) is a highly conserved melanogenic enzyme expressed in both pigmented and unpigmented melanomas of the mouse. To determine whether TRP-2 would be a good diagnostic marker for amelanotic melanomas of the dog, we performed immunohistochemistry for TRP-2, S-100, and Melan A on 21 canine tumors identified as amelanotic melanomas based on routine histopathologic examination. Thirteen of the tumors were TRP-2 positive, 10 were Melan A positive, and 19 were S-100 positive. TRP-2 was expressed in the cytoplasm of tumor cells in both primary and metastatic melanomas. S-100 staining was positive in all of three schwannomas and two of three gastrointestinal stromal tumors (one fibrosarcoma and one leiomyosarcoma) tested. Neither Melan A nor TRP-2 antibodies reacted with these tumors. Our findings indicate that staining for TRP-2 is a sensitive and specific method for confirming the diagnosis of amelanotic melanoma in dogs.

Key words: Dogs; immunohistochemistry; melan A protein; melanoma; S-100 proteins; tyrosinase-related protein-2.

Request reprints from Dr. D. F. Kusewitt, Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210 (USA). E-mail: kusewitt.1{at}osu.edu.




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[Abstract] [Full Text] [PDF]


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