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Vet Pathol 41:30-36 (2004)
© 2004 American College of Veterinary Pathologists

Osteoclast Numbers in Lewis Rats with Adjuvant-induced Arthritis: Identification of Preferred Sites and Parameters for Rapid Quantitative Analysis 1

B. Bolon, S. Morony, Y. Cheng, Y.-L. Hu and U. Feige

Departments of Pathology (BB 2), Metabolic Disease (SM), and Inflammation (YC, Y-LH, UF), Amgen, Inc. Thousand Oaks, CA

This study defined the best site for quantifying osteoclasts in male Lewis rats with mycobacteria-induced adjuvant arthritis. Hind paw sections of normal and arthritic rats (n = 6 per group) taken 7 days after disease onset were stained for osteoclasts using an anti–human cathepsin K primary antibody. Erosions and osteoclasts were assessed using semiquantitative scores (entire section) and quantitative measures (in calcaneus, navicular tarsal, and tibia). Bone area in arthritic rats was significantly reduced (P <= 0.05) by 39–55%, with the greatest decrease in the tarsal. Osteoclasts in arthritic rats were significantly increased (P <= 0.05) relative to normal in calcaneus (sevenfold), tarsal (194-fold), and tibia (threefold). The most useful quantitative indices were bone area and total osteoclasts, both defined as a percentage of total area measured. Semiquantitative scores for bone erosion and osteoclasts paralleled the quantitative indices. These data show that the navicular tarsal is the most sensitive site at which to assess arthritis in rats with this variant of adjuvant-induced arthritis.

Key words: Adjuvant-induced arthritis; cell count; osteoclast; rats.

Request reprints from Dr. B. Bolon, GEMpath, Inc., 2520 N. 400 W., Cedar City, UT 84720-8400 (USA). E-mail: bradgempath{at}aol.com.




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B. Bolon, G. Campagnuolo, L. Zhu, D. Duryea, D. Zack, and U. Feige
Interleukin-1{beta} and Tumor Necrosis Factor-{alpha} Produce Distinct, Time-dependent Patterns of Acute Arthritis in the Rat Knee
Vet. Pathol., May 1, 2004; 41(3): 235 - 243.
[Abstract] [Full Text] [PDF]


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