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Vet Pathol 41:37-43 (2004)
© 2004 American College of Veterinary Pathologists

Immunohistochemical Evaluation of Mononuclear Infiltrates in Canine Lupoid Onychodystrophy

R. S. Mueller, K. West and S. V. Bettenay

Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (RSM, SVB); and Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada (KW)

Claw biopsy samples of 11 dogs with lupoid onychodystrophy were evaluated. They were stained with hematoxylin and eosin and with antibodies against CD 3 as a T-cell marker, BLA 36 and HM 57 (CD 79{alpha}) as B-cell markers, and lysozyme, Mac 387, and major histocompatibility complex (MHC) class II as a marker for histiocytes using an immunoperoxidase and avidin–biotin technique. Inflammatory cells were counted in five high-power fields. The inflammatory infiltrate comprised predominantly B cells and T cells. Macrophages were typically only present in small numbers. CD 3, BLA 36, lysozyme, and MHC class II preserved significant antigenicity during formalin fixation and short decalcification for 24–48 hours, whereas CD 79{alpha} and particularly Mac 387 seemed to be more susceptible to denaturation by the decalcification process.

Key words: BLA 36; canine; CD 3; CD 79{alpha}; claw; immunohistochemistry; lysozyme; MHC class II; Mac 387.

Request reprints from Dr. R. S. Mueller, Medizinische Tierklinik, Ludwig-Maximilians-Universitaet Muenchen, Veterinaerstr 13, Meunchen, Germany. E-mail: ralf.mueller{at}med.vetmed.uni-muenchen.de.






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