This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Snyder, L. A. Articles by Moore, A. S. Search for Related Content PubMed PubMed Citation Articles by Snyder, L. A. Articles by Moore, A. S. Social Bookmarking                            What's this?

p53 Expression and Environmental Tobacco Smoke Exposure in Feline Oral Squamous Cell Carcinoma

Harrington Oncology Program (LAS, JJ-R, ASM) and Department of Pathology (RMJ), Tufts University School of Veterinary Medicine, North Grafton, MA; School of Public Health and Health Science, University of Massachusetts, Amherst, MA (ERB); and Cancer Center, University of Massachusetts, Worcester, MA (MSD)

The purpose of this study was to determine the prevalence of p53 overexpression in feline oral squamous cell carcinomas (SCC) and to determine, if any, the association between p53 overexpression and lifestyle factors and environmental exposures, including exposure to environmental tobacco smoke (ETS). Questionnaires concerning exposure to ETS and other environmental factors were sent to owners of cats presenting to the Harrington Oncology Program with a diagnosis of oral SCC between 1991 and 2000. Additionally, 23 formalin–fixed biopsy samples from these cats, with information regarding ETS, were evaluated immunohistochemically for p53 expression using the CM-1 clone and the avidin–biotin–horseradish peroxidase method. Of the 23 samples evaluated, 15 (65%) showed positive nuclear staining for the CM-1 clone. Tumor biopsy samples from cats exposed to any ETS were 4.5 times more likely to overexpress p53 than were tumors from unexposed cats (P = 0.19). Among cats with any ETS exposure, those with 5 years or longer of exposure were 7.0 times more likely to overexpress p53 (P = 0.38). Longhaired cats (P = 0.18) and female cats (P = 0.35) were also more likely to show p53 expression in their tumors. These results provide additional support for a relationship between oral SCC development and exposure to household ETS and may implicate p53 as a potential site for carcinogen–related mutation in this tumor.

Key words: Cancer; carcinogenesis; cats; environmental tobacco smoke; feline populations; immunohistochemistry; oral neoplasia; p53; squamous cell carcinoma; tumor suppressor gene.

Request reprints from Dr. A. S. Moore, Harrington Oncology Program, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536 (USA). antonymoore56{at}yahoo.co.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				U. Cardellino, Y. Ciribilli, V. Andreotti, P. Modesto, P. Menichini, G. Fronza, C. Pellegrino, and A. Inga Transcriptional properties of feline p53 and its tumour-associated mutants: a yeast-based approach Mutagenesis, November 1, 2007; 22(6): 417 - 423. [Abstract] [Full Text] [PDF]