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A von Willebrand's Factor Genomic Nucleotide Variant and Polymerase Chain Reaction Diagnostic Test Associated with Inheritable Type-2 von Willebrand's Disease in a Line of German Shorthaired Pointer Dogs

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA (JWK); and Departments of Microbiology and Molecular Genetics (PJV, VY-G) and Small Animal Clinical Sciences (PJV, SRK, YC, WDS, VY-G), College of Veterinary Medicine, Michigan State University, East Lansing, MI

Heritable, type-2 von Willebrand's disease (vWD) was studied in a line of German Shorthaired Pointers (GSPs) in which some members had a nucleotide variant in exon 28 of the von Willebrand factor (VWF) gene. A polymerase chain reaction (PCR) diagnostic test for the nucleotide variant was developed to establish the disorder's mode of inheritance and to eliminate it from the line. Thirty-six of the 49 GSPs in the line, 14 unrelated GSP controls, and 71 unrelated dogs of various breeds were tested for the presence of the variant nucleotide. All the dogs with a vWF antigen deficiency (<70% of normal) were either homozygous or heterozygous for the nucleotide variant. The variant was not located in any tested dog in the line or outside of the line with a vWF antigen value greater than 68%. Of the GSPs in the line tested, two were homozygous for the variant, 15 were heterozygous, and 19 were variant free. The collective evidence of this and other studies is consistent with the variant nucleotide being the cause of the type-2 vWD in this line of GSPs and German Wirehaired Pointers. The PCR diagnostic test for the variant nucleotide was successfully used to select and produce progeny that were variant free and vWD free. This test should be effective in the subsequent elimination of this same variant from other lines of dogs.

Key words: Coagulation; dogs; German Shorthaired Pointer; hemostasis; missense; mutation; vWD; vWF.

Request reprints from Dr. J. W. Kramer, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99163-6610 (USA). jwk{at}vetmed.wsu.edu.