This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Cho, W.-S. Articles by Chae, C. Search for Related Content PubMed PubMed Citation Articles by Cho, W.-S. Articles by Chae, C.

Expression of Cyclooxygenase-2 and Nitric Oxide Synthase 2 in Swine Ulcerative Colitis Caused by Salmonella typhimurium

Abstract

Cyclooxygenase-2 (COX-2) and nitric oxide synthase 2 (NOS2) were detected and localized in 20 pigs with ulcerative colitis caused by natural infection with Salmonella typhimurium. Evidence of NOS2 activity was determined by the formation of nitrotyrosine, a reaction product of peroxynitrite, in NOS2-expressing ulcerative colons by immunohistochemistry. Transcript RNA of COX-2 and NOS2 was consistently detected in colonic tissues from the 20 pigs with ulcerative colitis by using reverse transcription–polymerase chain reaction. Immunohistochemical signals for COX-2 and NOS2 were detected in the ulcerated area of all 20 pigs. Expression of COX-2 and NOS2 was identified continuously within inflammatory intestinal lesions but was minimal in unaffected regions of the colon of S. typhimurium–infected pigs. The immunohistochemistry of serial sections of intestine indicated that the majority of colons containing numerous COX-2–positive cells also had numerous NOS2-positive cells. Localization of NOS2 and a nitrotyrosine antigen was prominent in neutrophils and macrophages in the periphery of the lesions. Simultaneous detection of COX-2 and NOS2 RNA and protein indicated functional activity of prostaglandin and NO production in vivo. This study suggested that COX-2 and NOS2 expression may play a role in the pathophysiologic processes in ulcerative colitis caused by S. typhimurium.

Key words: Cyclooxygenase-2; immunohistochemistry; nitric oxide synthase 2; nitrotyrosine; pigs; Salmonella typhimurium; ulcerative colitis.

Request reprints from Dr. C. Chae, Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul (Republic of Korea). E-mail: swine{at}plaza.snu.ac.kr

This article has been cited by other articles:

				J. L. Gookin, J. Allen, S. Chiang, L. Duckett, and M. U. Armstrong Local Peroxynitrite Formation Contributes to Early Control of Cryptosporidium parvum Infection Infect. Immun., July 1, 2005; 73(7): 3929 - 3936. [Abstract] [Full Text] [PDF]