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Disseminated Encephalitozoonosis in Captive, Juvenile, Cotton-top (Saguinus oedipus) and Neonatal Emperor (Saguinus imperator) Tamarins in North America

ConZOOlting Wildlife Management, Barcelona, Spain (CJS, SS, LDA), Northwest ZooPath, Monroe, WA (MMG), Division of Microbiology, Tulane National Primate Research Center, Covington, LA (ESD, LCB), Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN (JARV), Electron Microscopy Laboratory, California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA (RWN), Africam Safari, Puebla, Pue., México (AP)

Disseminated encephalitozoonosis was diagnosed in 2 sibling, juvenile, cotton-top tamarins (Saguinus oedipus) and 3 sibling, neonatal, emperor tamarins (S. imperator) by use of histologic examination, histochemical analysis, electron microscopy, and polymerase chain reaction (PCR) analysis with nucleotide sequencing. All tamarins were captive born at zoos in North America and died with no premonitory signs of disease. The main pathologic findings were myocarditis (4/5), hepatitis (3/5), interstitial pneumonia (3/5), skeletal myositis (3/5), meningoencephalitis (2/5), adrenalitis (2/5), tubulointerstitial nephritis (1/5), myelitis (1/5), sympathetic ganglioneuritis (1/5), and retinitis (1/5). Central nervous system lesions were the most prominent findings in cotton-top tamarins. The inflammation was predominantly lymphocytic and suppurative in cotton-top tamarins, whereas emperor tamarins had granulomatous or lymphoplasmacytic lesions. Intralesional periodic acid–Schiff-, gram-, or acid–fast (or all 3)-positive, oval-to-elliptical shaped organisms were found in 1 cotton-top and the 3 emperor tamarins. By electron microscopy, these organisms were consistent with microsporidia of the genus Encephalitozoon. E. cuniculi genotype III was detected by PCR analysis and sequencing in paraffin-embedded brain, lung, and bone marrow specimens from the cotton-top tamarins. Although PCR results were negative for one of the emperor tamarins, their dam was seropositive for E. cuniculi by ELISA and Western blot immunodetection. These findings and recent reports of encephalitozoonosis in tamarins in Europe suggest that E. cuniculi infection may be an emerging disease in callitrichids, causing high neonatal and juvenile mortality in some colonies. The death of 2 less than 1-day-old emperor tamarins from a seropositive dam supports the likelihood of vertical transmission in some of the cases reported here.

Key words: Encephalitis; Encephalitozoon cuniculi; hepatitis; microsporidiosis; nephritis; New World primate; polymerase chain reaction; tamarin.

Request reprints from Carles Juan-Sallés, Department of Pathology, ConZOOlting Wildlife Management, Serra del Montsant 6, 08415 Bigues i Riells, Barcelona, (Spain). E-mail: patologia{at}conzoolting.com