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Vet Pathol 43:896-903 (2006)
© 2006 American College of Veterinary Pathologists

The Immunohistochemical Characterization of Devil Facial Tumor Disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii)

R. Loh1, D. Hayes2, A. Mahjoor3, A. O'Hara2, S. Pyecroft1 and S. Raidal2

1 Animal Health Laboratory, Tasmania, Australia (RL, DH, SP), 2 Division of Veterinary & Biomedical Sciences, Murdoch University, Western Australia (AO, SR), 3 and Department of Pathobiology, School of Veterinary Medicine, Shiraz, IRAN (AM)

Immunohistochemical techniques were used to characterize the disfiguring and debilitating fatal neoplastic disease, devil facial tumor disease (DFTD), which has recently affected a significant proportion of the wild population of Tasmanian Devils (Sarcophilus harrisii). The diagnostic values of a number of immunohistochemical stains were employed to further characterize 50 representative cases. The neoplasms were negative for cytokeratin (0/48), epithelial membrane antigen (0/42), von Willebrand factor (vWF) (0/11), smooth muscle actin (SMA) (0/26), desmin (0/47), glial fibrillary acid protein (0/13), CD16 (0/13), CD57 (0/43), CD3 (0/18), and LSP1 (0/16). DFTD cells were positive for vimentin (50/50), S-100 (41/48), melan A (11/39), neuron specific enolase (35/35), chromogranin A (12/12) and synaptophysin (29/30). The cells were negative for amyloid (0/30) and stained negatively with Singh's silver (0/34) but were weakly argyrophilic (3/40) using Grimelius histochemical stain. These staining characteristics are consistent with cells of neuroectodermal origin.

Key words: Chromogranin A; devil facial tumor disease; immunohistochemistry; melan A; NSE; S-100; Sarcophilus harrisii; synaptophysin; Tasmanian Devils; vimentin.

Request reprints from Richmond Loh, Animal Health Laboratory, PO Box 46, Kings Meadows 7249, Tasmania (Australia). E-mail: Richmond.Loh{at}dpiwe.tas.gov.au




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[Abstract] [Full Text] [PDF]


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