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Experimental Transmission and Induction of Ranaviral Disease in Western Ornate Box Turtles (Terrapene ornata ornata) and Red-Eared Sliders (Trachemys scripta elegans)

Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL (AJJ, ERJ), and Division of Pathology, Conservation and Research for Endangered Species, Zoological Society of San Diego, San Diego, CA (APP)

An experimental transmission study was designed to determine whether a causal relationship exists between a Ranavirus (BSTRV) isolated from a Burmese star tortoise that died and the lesions observed in that tortoise. A pilot study was performed with 3 box turtles (Terrapene ornata ornata) and 3 red-eared sliders (RESs; Trachemys scripta elegans) to assess their suitability in a larger study. Based on the outcome of this study, RESs were selected, and 2 groups of 4 RESs received either an oral (PO) or intramuscular (IM) inoculum containing10⁵ 50% Tissue Culture Infecting Dose (TCID₅₀) of a BSTRV-infected cell lysate. One turtle each was mock inoculated PO or IM with the same volume of uninfected cell lysate. Three of four IM-inoculated RESs developed clinical signs (nasal and ocular discharge [3 of 3], oral plaques [1 of 3], conjunctivitis and hyphema [1 of 3] and extreme lethargy [3 of 3]). A Ranavirus was isolated from kidney homogenates of 3 euthanatized turtles; DNA sequences of a portion of the major capsid protein gene were amplified by polymerase chain reaction. Consistent histologic lesions were observed only in IM-inoculated turtles and included fibrinoid vasculitis centered on splenic ellipsoids, multifocal hepatic necrosis, and multicentric fibrin thrombi in a variety of locations, including hepatic sinusoids, glomerular capillary loops, and pulmonary capillaries. Virions compatible with Ranavirus were observed within necrotic cells of the spleen of 1 IM-inoculated turtle using transmission electron microscopy. This study fulfills Koch's postulates, confirming a causal relationship between BSTRV and the clinical and histologic changes in chelonians infected with this virus.

Key words: Iridovirus; pathology; Ranavirus; reptile; transmission; turtle.

Request reprints from E. R. Jacobson, SACS, Box 100126, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610 (USA). E-mail: JacobsonE{at}mail.vetmed.ufl.edu

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